A multi-center human factors validation study using the IKE Age Verification System was conducted with 102 participants (51% male, 49% female) aged 18-67 (22% 18-20 years, 18% 21-24 years, 20% 25-44 years, 20% 45-64 years, 20% 65 years or older) who had smartphones and were comfortable using smartphone applications. This study aimed to validate the design and development and to test a smartphone-based application accuracy at age verification, and a system-on-a-chip embedded into a device effectiveness at activating/deactivating the device based on a user’s age. Participants underwent a task-based usability assessment to test the function and effectiveness of the IKE System followed by an Ease-of-Use scale assessment to measure the satisfaction, interface effectiveness, and overall system usability. The study evaluated the IKE System with the intended-to-market user interface and representative users in representative simulated-use environments. All participants that downloaded the IKE application (n=101), were able to accurately age verify, and the device was only activated for those of legal age or older. All (100%) devices were deactivated after 15 minutes of idle time, and after losing Bluetooth signal (out of range). Users were able to re-connect and re-activate their devices. Age, race/ethnicity, and gender did not impact identification or age verification. Participant ranked ease-of-use of the IKE application was 90% Very Easy or Extremely Easy, 8% Somewhat Easy, and 2% Somewhat Easy or Not Easy. User errors were rare (1%, n=102) with simulated use, with only one participant unable to download the application.